CaptureELISA
實驗概要Capture ELISA (also known as "sandwich" ELISA) is a sensitive assay to quantitate picogram to microgram quantities of substances (such as hormones, cell signaling chemicals, infectious disease antigens and cytokines.). This type of ELISA design may be needed rather than a direct or indirect ELISA for several reasons. The substance to be analyzed may be too dilute to b......閱讀全文
Capture－ELISA
實驗概要Capture ELISA ?(also known as "sandwich" ELISA) is a sensitive assay to quantitate ?picogram to microgram quantities of substances (such as hormon
Actin－Capture－Assay
David AmbergDialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 .Mix 5ug actin into 50ul total volume binding buffer.Mix 5ug GST-fusio
Laser－Capture－Microdissection－(LCM)
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
ImmunoLaser－Capture－Microdissection
A: Development of Immuno-LCMLimitation of MicrodissectionMicrodissection of routinely stained or unstained frozen sections has been used successfully
電子捕獲檢測器(electron－capture－detector,ECD)結構
檢測室內有正負電極與β-射線源，目前所使用的最佳的放射源是Ni63，在衰變中沒有γ輻射，產生的β射線能量低，半衰期長，可用到400℃。
電子捕獲檢測器(electron－capture－detector,ECD)原理
檢測室內的放射源放出β-射線粒子(初級電子)，與通過檢測室的載氣碰撞產生次級電子和正離子，在電場作用下，分別向與自己極性相反的電極運動，形成檢測室本底電流，當具有負電性的組分(即能捕獲電子的組分)進入檢測室后，捕獲了檢測室內的電子，變成帶負電荷的離子，由于電子被組分捕獲，使得檢測室本底電流減少，
ELISA
Gangliosides ELISA protocol?(Contributed by?pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
Sandwich－ELISA－Protocol
實驗概要The ?Sandwich ELISA measures the amount of antigen between two layers of ?antibodies (i.e. capture and detection antibody). The antigen to be ?mea
夾心ELISA1
Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or
好消息PeproTech－ELISA操作指南視頻已上傳
? ? ?美國PeproTech (派普泰克)?公司是世界上領先的重組細胞因子和蛋白生產商，同時也生產多種細胞因子和蛋白的ELISA法檢測試劑盒，詳細信息請查看www.peprotech.com，或與PeproTech中國代表處?(電話：0512-6832 5993，Email：china@pepr
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
解決非特異吸附的新方法
免疫分析中，非特異的蛋白吸附常常是阻礙高靈敏度的一個重要的因素。來自中科院等單位的研究小組開發了一種新的固相載體，有望達到"0"非特異吸附。以下是他們在 Analytical Chemistry 的文章摘要。Integrated Poly(dimethysiloxane) with an Intri
COMPETITION－ELISA
The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be des
COMPETITION－ELISA
實驗概要? ? ? ? The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and
登革熱IgM－ELISA試劑盒說明書(捕獲法)（六）
回收率研究五天內在不同的三個地點,兩個不同的操作者完成對登革熱 IgM Capture ELISA檢測試劑盒可重現性研究實驗的評估.樣本(包括質控)均同時采用三份檢測.實驗分別在佛羅里達州一間公共健康實驗室, InBios,美國中部的一家實驗室.此次研究實驗采用4份血清樣本(均按照相關規定稀
ELISPOT－(Enzymelinked－ImmunoSPOT)－實驗方法步驟1
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
登革熱IgM－ELISA試劑盒說明書(捕獲法)（五）
研究中心3：登革熱IgM Capture ELISA試劑盒特異性的評估在美國中西部的一間公共健康實驗室完成.此項研究采用了289份有登革熱癥狀樣本(2005-2008年期間收集) (其中136位個體并發患有西尼羅河病毒, 甲型肝炎,乙型肝炎,HIV, 落磯山斑點熱(RMSF), 軍團病或
ELISA
ELISA是一種經典的測定液體樣本中蛋白含量的方法。該方法優點在于可以準確定量蛋白含量，且測定樣本的種類可以多種多樣。本期小編帶你一起了解ELISA不同樣品的制備方法。細胞培養上清液移取細胞培養基至離心管中，4℃ 下以 1500 rpm 的速度離心 10 分鐘。立即分裝上清液，并將樣品儲存在 -80
ELISA
實驗概要ELISA是酶聯接免疫吸附劑測定( Enzyme-Linked Immunosorbnent Assay )的簡稱。它是繼免疫熒光和放射免疫技術之后發展起來的一種免疫酶技術。此項技術自70年代初問世以來，發展十分迅速，目前已被廣泛用于生物學和醫學科學的許多領域。實驗原理ELISA是以
ELISA:－Sandwich
實驗概要The following protocol provides a method of sandwich ELISA by Peprotech.主要試劑Tween-20 (Sigma Cat. # P-7949); BSA (Sigma Cat. # A-7030); ABTS Liqu
ELISPOT－(Enzymelinked－ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
ELISA實驗
? ? ? ? ? ? 實驗方法原理 ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。 實驗材料
ELISA－with－Platelet
OUTLINEThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies o
ELISA實驗
實驗材料 抗原血清試劑、試劑盒 碳酸鹽包被緩沖液PBSTBSA儀器、耗材 96孔酶標板4℃冰箱恒溫培養箱分光光度計實驗步驟 一、包被抗原?1. 用50mM 的碳酸鹽包被緩沖液（pH 9.6）溶解抗原，使抗原濃度為10-20 μg/ml，加100 μl/孔到96 孔酶標板，4℃放置過夜。2. 第二天棄
ELISA－protocol
ELISA protocol：1.取5－10ul BMMY表達上清用0.05M NaHCO3稀釋到100ul鋪ELISA板，37度或室溫振蕩大于1小時。注意一定要做一個GS115空菌株表達上清作為陰性對照，最好還找一個帶有histag的蛋白作為陽性對照。2.TPBS洗板3次，方法：倒掉鋪板液，倒置于
ELISA實驗
酶聯免疫吸附實驗（ELISA）可以用于：（1）檢測大分子抗原和特異性抗體等；（2）具有快速、靈敏、簡便、載體易于標準化等優點。實驗方法原理ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。
In－Cell－ELISA
實驗概要This protocol is a general protocol for ICE analysis and can be used with any of Abcam's ICE-validated antibodies. Antibodies are availabl
ELISA:－Direct
實驗概要The following protocol provides a method of direct ELISA by Peprotech.主要試劑Tween-20 (Sigma Cat. # P-7949) BSA (Sigma Cat. # A-7030)? ABTS Liquid Su
Competitive－ELISA
DAY 11. Coat Nunc immuno-module plates overnight, in usual manner.2. Set up competition assay between antibody and competing substance :-Prepare a 1:2